Targeted genome editing across species using ZFNs and TALENs.
نویسندگان
چکیده
Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.
منابع مشابه
TALENs facilitate targeted genome editing in human cells with high specificity and low cytotoxicity
Designer nucleases have been successfully employed to modify the genomes of various model organisms and human cell types. While the specificity of zinc-finger nucleases (ZFNs) and RNA-guided endonucleases has been assessed to some extent, little data are available for transcription activator-like effector-based nucleases (TALENs). Here, we have engineered TALEN pairs targeting three human loci ...
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1Center for Genome Engineering, University of Minnesota, Minneapolis, Minnesota, USA; 2Department of Animal Science, University of Minnesota, Saint Paul, Minnesota, USA; 3Department of Genetics, Cell Biology and Development, Minneapolis, Minnesota, USA Correspondence: Perry B Hackett, Department of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, US...
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عنوان ژورنال:
- Science
دوره 333 6040 شماره
صفحات -
تاریخ انتشار 2011